Sample Set Annotation Tool: Genome-wide analysis of B lymphocytes derived from human pluripotent stem cells, neonatal and adult sources

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

The aim of the array was to compare iPS-derived CD19+CD10+ B cells with B cells from umbilical cord blood (UCB) and adult peripheral blood (PB)

Hypothesis

Experimental Design

iPS-CD34+ cells and UCB-CD133+ cells were differentiated to the B cell lineage in vitro. B cells were also isolated directly from UCB and PB. RNA was extracted from CD19+CD10+ FACS isolated cells from all 4 B cell samples as well as from undifferentiated iPS cells.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

19 Samples Loaded: 19

Species

Human (Homo sapiens)

Sample Source

Adult , B cells , Neonatal

Disease

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	Cell type	Group name	Cell source	In vitro culture
GSM1296287	iPS_1	B cell	iPS-CD34+ derived B cells	iPS	Yes
GSM1296288	iPS_2	B cell	iPS-CD34+ derived B cells	iPS	Yes
GSM1296289	iPS_3	B cell	iPS-CD34+ derived B cells	iPS	Yes
GSM1296290	iPS_4	B cell	iPS-CD34+ derived B cells	iPS	Yes
GSM1296291	non_1	iPS	iPS cells	iPS	Yes

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File

GSE53572 modified.csv Sample Set Spreadsheet
GSE53572 modified.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Cell type	Group name	Cell source	In vitro culture
GSM1296287	iPS_1	B cell	iPS-CD34+ derived B cells	iPS	Yes
GSM1296288	iPS_2	B cell	iPS-CD34+ derived B cells	iPS	Yes
GSM1296289	iPS_3	B cell	iPS-CD34+ derived B cells	iPS	Yes
GSM1296290	iPS_4	B cell	iPS-CD34+ derived B cells	iPS	Yes
GSM1296291	non_1	iPS	iPS cells	iPS	Yes
GSM1296292	non_2	iPS	iPS cells	iPS	Yes
GSM1296293	non_3	iPS	iPS cells	iPS	Yes
GSM1296294	non_4	iPS	iPS cells	iPS	Yes
GSM1296295	HSC_1	B cell	UCB-CD133+ derived B cells	UCB-CD133+	Yes
GSM1296296	HSC_2	B cell	UCB-CD133+ derived B cells	UCB-CD133+	Yes
GSM1296297	HSC_3	B cell	UCB-CD133+ derived B cells	UCB-CD133+	Yes
GSM1296298	HSC_4	B cell	UCB-CD133+ derived B cells	UCB-CD133+	Yes
GSM1296299	UCB_1	B cell	UCB B cells	UCB	No
GSM1296300	UCB_2	B cell	UCB B cells	UCB	No
GSM1296301	UCB_3	B cell	UCB B cells	UCB	No
GSM1296302	UCB_4	B cell	UCB B cells	UCB	No
GSM1296303	PB_1	B cell	PB B cells	PB	No
GSM1296304	PB_2	B cell	PB B cells	PB	No
GSM1296305	PB_4	B cell	PB B cells	PB	No

Group Sets View in Gene Expression Browser

Name
All Samples	Default	View
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Module Analysis

Group Set
All Samples

SIDRA