Sample Set Annotation Tool: Expression data from healthy human CD161++CD8aa and CD161++CD8ab T cells

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Hypothesis

We used microarrays to compare gene expression between healthy human CD161++CD8aa and CD161++CD8ab T cells.

Experimental Design

We sorted freshly obtained peripheral blood mononucleocytes from healthy controls by first using magnetic beads to negatively select for CD8a+ T cells, fluorescently labelling isolated cells with antibodies and subsequent sorting using MoFlow MLS cell sorter (DAKO). Sorted samples were snap frozen. RNA was later extracted from the sorted populations prior to hybridization to Affymetrix arrays.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

8 Samples Loaded: 8

Species

Human (Homo sapiens)

Sample Source

Neonatal , Adult , CD8+ T cells

Disease

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	tissue	Cell type	Disease state	Group name
GSM825517	CD161++CD8aa T cells, biological rep 1	peripheral blood	CD161++CD8aa T cells T cells	healthy	CD161++CD8aa T cells
GSM825518	CD161++CD8ab T cells, biological rep 1	peripheral blood	CD161++CD8ab T cells T cells	healthy	CD161++CD8ab T cells
GSM825519	CD161++CD8aa T cells, biological rep 2	peripheral blood	CD161++CD8aa T cells T cells	healthy	CD161++CD8aa T cells
GSM825520	CD161++CD8ab T cells, biological rep 2	peripheral blood	CD161++CD8ab T cells T cells	healthy	CD161++CD8ab T cells
GSM825521	CD161++CD8aa T cells, biological rep 3	peripheral blood	CD161++CD8aa T cells T cells	healthy	CD161++CD8aa T cells

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File

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Sample ID	!Sample Title	Tissue	Cell type	Disease state	Group name
GSM825517	CD161++CD8aa T cells, biological rep 1	peripheral blood	CD161++CD8aa T cells T cells	healthy	CD161++CD8aa T cells
GSM825518	CD161++CD8ab T cells, biological rep 1	peripheral blood	CD161++CD8ab T cells T cells	healthy	CD161++CD8ab T cells
GSM825519	CD161++CD8aa T cells, biological rep 2	peripheral blood	CD161++CD8aa T cells T cells	healthy	CD161++CD8aa T cells
GSM825520	CD161++CD8ab T cells, biological rep 2	peripheral blood	CD161++CD8ab T cells T cells	healthy	CD161++CD8ab T cells
GSM825521	CD161++CD8aa T cells, biological rep 3	peripheral blood	CD161++CD8aa T cells T cells	healthy	CD161++CD8aa T cells
GSM825522	CD161++CD8ab T cells, biological rep 3	peripheral blood	CD161++CD8ab T cells T cells	healthy	CD161++CD8ab T cells
GSM825523	CD161++CD8aa T cells, biological rep 4	peripheral blood	CD161++CD8aa T cells T cells	healthy	CD161++CD8aa T cells
GSM825524	CD161++CD8ab T cells, biological rep 4	peripheral blood	CD161++CD8ab T cells T cells	healthy	CD161++CD8ab T cells

Group Sets View in Gene Expression Browser

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Module Analysis

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