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In Progress
Clinical

GSE30032

Purpose

Passive smoke intake by pregnant women may have detrimental effects such as spontaneous abortion, lower birth weight, stillbirth, and reduced infant lung function. To extend our knowledge on molecular effects of tobacco smoke exposure in pregnancy, we analyzed transcriptome alterations in passive smokers (PS) and compared them to those in active smokers (AS). Using Illumina Expression Beadchip with 24,526 transcript probes, gene expression patterns were assayed in placentas from PS (N=25) exposed to environmental tobacco smoke (ETS) throughout pregnancy and non-exposed (NS) counterparts (N=35), and in cord blood cells from their newborns. The ETS exposure was evaluated by questionnaire disclosure and cotinine measurement in maternal and cord bloods. A total of 196 genes were significantly deregulated in placentas of PS compared to NS. These genes were primary associated with extracellular matrix, apoptosis, blood clotting, response to stress, embryonic morphogenesis, and lipid metabolism. Cord blood of newborns of PS displayed differential expression of 116 genes encoding mainly neuronal factors, regulators of immunologic response, and protooncogenes. Gene ontology analyses highlighted some important biological processes that might be associated with placental insufficiency and fetal growth restriction in PS, such as fatty acid catabolism, coagulation, regulation of growth, and response to steroid hormone stimulus. The study demonstrates that even low dose exposure to ETS during pregnancy leads to the significant deregulation of transcriptional regulation in placental and fetal cells. The data suggest the effect of ETS on the fetus is primary indirect, mediated via deregulation of placental functions. Comparison of PS and AS indicated that ETS exposure and active smoking in pregnancy partly employ the same molecular mechanisms.

Hypothesis

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Experimental Design

A total of 60 women who gave birth to a baby in the Faculty Hospital Motol (Czech Republic) were enrolled into the study approved by the local Institutional Review Board. The participants signed written informed consent and self-reported information on tobacco smoke exposure (type of exposure, number of cigarettes per day, exposure period) and life style during pregnancy (alcohol drinking, diet, area of residency etc.). Further, the women completed the questionnaire on pregnancy/delivery course (complications, diseases, medication, and delivery mode) and newborn characteristics (physical parameters, Apgar score) with the assistance of gynecologist. Only women without any health complications during pregnancy and their newborns without manifestation of pathological abnormalities were included in the study. Based on self-reported tobacco smoke exposure, the women were divided into two groups, non-smokers (NS, N=35) and passive smokers (PS, N=25). Former and active smokers were excluded from the study. Exposure to tobacco smoke was further evaluated by measurement of plasma cotinine levels in maternal peripheral blood and newborn cord blood using radioimmunoassay.

Experimental Variables

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Controls

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Methods

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Additional Information

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Microarray
Illumina HumanRef-8 v3
104 Samples Loaded: 104
Human (Homo sapiens)
Whole Blood , Placenta
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample Title parity Age (years) Apgar score (5s) Baby's sex
GSM743216 passive smoker 201, placenta 6 32 8 F
GSM743217 passive smoker 203, placenta 5 38 10 F
GSM743218 non-smoker 204, placenta 1 30 10 M
GSM743219 non-smoker 205, placenta 1 33 10 M
GSM743220 passive smoker 206, placenta 4 33 10 F
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File

GSE30032 modified.csv
Sample Set Spreadsheet
Log2 Transformed
Raw Signal
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Clinical Datasource
Links

Samples Viewer / Editor

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Sample ID !Sample Title Parity Age (years) Apgar score (5s) Baby's sex Cord blood cotinin (ng/ml) Gestational age (weeks) Maternal blood cotinin (ng/ml) Maternal bmi Mode of delivery Newborn length (cm) Newborn weight (g) Placental volume (cm3) Placental weight (g) Smoking status Group name
GSM743216
passive smoker 201, placenta
6
32
8
F
1.1
39
0.84
29
vaginal
50
3540
1188
680
passive smoker
placenta passive smoker
GSM743217
passive smoker 203, placenta
5
38
10
F
0.12
40
0.11
28
caesarean
51
4130
1080
650
passive smoker
placenta passive smoker
GSM743218
non-smoker 204, placenta
1
30
10
M
0.15
39
0.14
23
vaginal
50
3370
1020
690
non-smoker
placenta non-exposed
GSM743219
non-smoker 205, placenta
1
33
10
M
0.15
37
0.13
20
caesarean
48
2870
1080
720
non-smoker
placenta non-exposed
GSM743220
passive smoker 206, placenta
4
33
10
F
0.18
38
0.09
25
caesarean
49
3520
1500
unknown
passive smoker
placenta passive smoker
GSM743221
passive smoker 207, placenta
1
27
9
F
0.24
38
0.1
19
vaginal
46
2850
648
400
passive smoker
placenta passive smoker
GSM743222
non-smoker 208, placenta
2
35
10
M
0.4
40
0.21
25
vaginal
48
3390
420
670
non-smoker
placenta non-exposed
GSM743223
passive smoker 209, placenta
2
30
10
F
0.2
39
0.11
26
vaginal
50
3830
880
580
passive smoker
placenta passive smoker
GSM743224
non-smoker 210, placenta
2
29
10
M
0.15
41
0.12
19
vaginal
51
3440
1188
680
non-smoker
placenta non-exposed
GSM743225
non-smoker 211, placenta
1
30
10
M
0.53
40
0.26
21
vaginal
50
3680
1280
700
non-smoker
placenta non-exposed
GSM743226
passive smoker 212, placenta
1
35
10
F
0.11
39
0.09
21
vaginal
49
3360
1875
600
passive smoker
placenta passive smoker
GSM743227
passive smoker 215, placenta
2
34
10
M
no sample
38
0.46
24
vaginal
43
2270
1500
620
passive smoker
placenta passive smoker
GSM743228
non-smoker 218, placenta
1
27
10
F
0.21
37
0.15
24
vaginal
48
2820
1725
620
non-smoker
placenta non-exposed
GSM743229
passive smoker 219, placenta
1
22
10
F
1.4
39
1.32
17
vaginal
49
2610
1950
700
passive smoker
placenta passive smoker
GSM743230
non-smoker 220, placenta
5
38
10
F
0.3
41
0.27
20
vaginal
53
4170
1188
750
non-smoker
placenta non-exposed
GSM743231
non-smoker 222, placenta
1
30
10
M
0.28
42
0.2
21
caesarean
52
3730
720
650
non-smoker
placenta non-exposed
GSM743232
passive smoker 225, placenta
1
30
10
M
1.31
38
1.24
18
vaginal
48
2970
864
600
passive smoker
placenta passive smoker
GSM743233
non-smoker 226, placenta
2
31
9
F
0.17
41
0.16
20
vaginal
51
3570
864
650
non-smoker
placenta non-exposed
GSM743234
non-smoker 228, placenta
1
34
10
F
0.09
41
0.09
18
vaginal
45
2810
2100
600
non-smoker
placenta non-exposed
GSM743235
non-smoker 231, placenta
3
32
9
F
0.16
40
0.12
29
vaginal
50
3650
1500
750
non-smoker
placenta non-exposed
GSM743236
passive smoker 232, placenta
2
34
10
F
0.36
40
0.49
37
vaginal
49
3170
1452
650
passive smoker
placenta passive smoker
GSM743237
passive smoker 234, placenta
1
24
10
M
0.29
40
0.23
23
vaginal
48
3200
1440
600
passive smoker
placenta passive smoker
GSM743238
non-smoker 236, placenta
1
33
10
F
0.28
39
0.09
27
vaginal
48
2980
1120
620
non-smoker
placenta non-exposed
GSM743239
non-smoker 238, placenta
2
32
10
M
0.13
41
0.11
21
vaginal
53
3950
2700
670
non-smoker
placenta non-exposed
GSM743240
non-smoker 240, placenta
3
31
10
F
0.23
38
0.33
17
vaginal
50
2880
576
620
non-smoker
placenta non-exposed
GSM743241
non-smoker 241, placenta
2
28
10
M
0.1
41
0.08
21
vaginal
46
2860
972
650
non-smoker
placenta non-exposed
GSM743242
passive smoker 245, placenta
6
31
10
M
1.18
39
1.45
22
vaginal
51
3600
1760
690
passive smoker
placenta passive smoker
GSM743243
passive smoker 246, placenta
1
27
9
M
0.16
41
0.13
20
caesarean
51
3370
1200
630
passive smoker
placenta passive smoker
GSM743244
non-smoker 248, placenta
1
35
10
F
0.15
40
0.12
26
vaginal
53
3690
1320
600
non-smoker
placenta non-exposed
GSM743245
passive smoker 251, placenta
2
34
8
M
0.34
36
0.14
19
caesarean
47
2840
1500
580
passive smoker
placenta passive smoker
GSM743246
passive smoker 256, placenta
1
26
10
M
1.57
41
1.09
21
vaginal
49
3500
1875
730
passive smoker
placenta passive smoker
GSM743247
passive smoker 258, placenta
3
32
10
F
1.76
35
1.41
26
vaginal
48
3490
1152
unknown
passive smoker
placenta passive smoker
GSM743248
non-smoker 259, placenta
1
31
10
F
0.25
39
0.17
21
vaginal
53
3690
972
720
non-smoker
placenta non-exposed
GSM743249
non-smoker 260, placenta
2
31
10
F
0.2
38
0.19
19
vaginal
48
3460
448
unknown
non-smoker
placenta non-exposed
GSM743250
non-smoker 261, placenta
2
32
10
F
0.16
38
0.13
28
vaginal
53
3940
3150
unknown
non-smoker
placenta non-exposed
GSM743251
passive smoker 262, placenta
2
26
9
M
0.26
39
0.38
22
caesarean
55
4550
1656
unknown
passive smoker
placenta passive smoker
GSM743252
passive smoker 263, placenta
2
31
8
F
1.36
37
1.41
24
vaginal
48
2650
720
unknown
passive smoker
placenta passive smoker
GSM743253
non-smoker 265, placenta
1
24
10
M
0.26
40
0.14
30
vaginal
51
3530
800
unknown
non-smoker
placenta non-exposed
GSM743254
non-smoker 266, placenta
2
32
10
F
0.23
38
0.3
18
vaginal
51
3250
1026
700
non-smoker
placenta non-exposed
GSM743255
passive smoker 267, placenta
6
35
10
F
0.27
42
0.16
22
caesarean
50
3080
1140
500
passive smoker
placenta passive smoker
GSM743256
non-smoker 268, placenta
2
32
10
F
0.25
40
0.2
19
vaginal
49
3040
1134
unknown
non-smoker
placenta non-exposed
GSM743257
passive smoker 270, placenta
1
34
9
M
1.46
39
0.95
34
caesarean
49
3370
1875
400
passive smoker
placenta passive smoker
GSM743258
passive smoker 274, placenta
1
24
9
M
0.25
39
0.2
20
vaginal
51
3400
880
unknown
passive smoker
placenta passive smoker
GSM743259
non-smoker 275, placenta
2
30
10
M
0.11
40
0.14
20
vaginal
51
3650
1500
640
non-smoker
placenta non-exposed
GSM743260
non-smoker 282, placenta
2
21
10
M
0.21
40
0.23
21
vaginal
49
3260
1800
unknown
non-smoker
placenta non-exposed
GSM743261
non-smoker 284, placenta
2
27
10
F
0.23
36
0.11
29
vaginal
48
2870
1000
unknown
non-smoker
placenta non-exposed
GSM743262
non-smoker 287, placenta
1
30
10
M
0.31
39
0.12
21
vaginal
47
2620
1071
unknown
non-smoker
placenta non-exposed
GSM743263
passive smoker 289, placenta
unknown
27
9
F
0.27
41
0.19
21
vaginal
53
4150
1188
unknown
passive smoker
placenta passive smoker
GSM743264
non-smoker 291, placenta
1
29
10
M
0.44
39
0.29
21
vaginal
49
3040
864
unknown
non-smoker
placenta non-exposed
GSM743265
non-smoker 292, placenta
2
36
10
M
0.65
38
0.8
22
vaginal
46
2890
800
unknown
non-smoker
placenta non-exposed
GSM743266
non-smoker 294, placenta
3
35
10
M
0.12
40
0.12
22
vaginal
53
4720
1080
unknown
non-smoker
placenta non-exposed
GSM743267
passive smoker 295, placenta
3
30
10
F
0.24
37
0.31
26
vaginal
49
3720
640
700
passive smoker
placenta passive smoker
GSM743268
non-smoker 296, placenta
1
30
10
F
0.2
41
0.12
22
vaginal
53
3770
972
unknown
non-smoker
placenta non-exposed
GSM743269
non-smoker 297, placenta
6
33
10
M
0.28
38
0.29
29
vaginal
52
3240
864
unknown
non-smoker
placenta non-exposed
GSM743270
non-smoker 298, placenta
1
30
10
F
0.28
37
0.18
22
caesarean
49
3250
864
unknown
non-smoker
placenta non-exposed
GSM743271
non-smoker 299, placenta
1
30
10
M
0.16
41
0.12
20
vaginal
50
3770
1794
unknown
non-smoker
placenta non-exposed
GSM743272
non-smoker 300, placenta
2
30
9
M
0.36
39
0.29
21
vaginal
47
2570
810
unknown
non-smoker
placenta non-exposed
GSM743273
passive smoker 201, cord blood
6
31.78370979
8
F
1.096248128
39
0.839590006
28.76397107
vaginal
50
3540
1188
680
passive smoker
cord blood passive smoker
GSM743274
passive smoker 203, cord blood
5
38.31074606
10
F
0.115202267
40
0.108987726
27.51338489
caesarean
51
4130
1080
650
passive smoker
cord blood passive smoker
GSM743275
non-smoker 204, cord blood
1
29.80150582
10
M
0.147580246
39
0.144795088
23.183391
vaginal
50
3370
1020
690
non-smoker
cord blood non-exposed
GSM743276
passive smoker 209, cord blood
2
30.20396988
10
F
0.196401244
39
0.11
25.86451247
vaginal
50
3830
880
580
passive smoker
cord blood passive smoker
GSM743277
non-smoker 210, cord blood
2
29.12251882
10
M
0.154044299
41
0.118542103
19.48696145
vaginal
51
3440
1188
680
non-smoker
cord blood non-exposed
GSM743278
non-smoker 211, cord blood
1
29.62628337
10
M
0.53
40
0.26
20.9572742
vaginal
50
3680
1280
700
non-smoker
cord blood non-exposed
GSM743279
passive smoker 212, cord blood
1
34.65845311
10
F
0.11
39
0.09
20.90419501
vaginal
49
3360
1875
600
passive smoker
cord blood passive smoker
GSM743280
passive smoker 215, cord blood
2
34.49691992
10
M
no sample
38
0.46
23.7953599
vaginal
43
2270
1500
620
passive smoker
cord blood passive smoker
GSM743281
non-smoker 218, cord blood
1
26.87474333
10
F
0.210152083
37
0.149427099
23.73866213
vaginal
48
2820
1725
620
non-smoker
cord blood non-exposed
GSM743282
passive smoker 219, cord blood
1
21.90554415
10
F
1.398597354
39
1.322443314
16.80613424
vaginal
49
2610
1950
700
passive smoker
cord blood passive smoker
GSM743283
non-smoker 222, cord blood
1
30.36002738
10
M
0.276631334
42
0.2
20.54863176
caesarean
52
3730
720
650
non-smoker
cord blood non-exposed
GSM743284
passive smoker 225, cord blood
1
29.83983573
10
M
1.313161625
38
1.235355728
18.02595738
vaginal
48
2970
864
600
passive smoker
cord blood passive smoker
GSM743285
non-smoker 226, cord blood
2
31.23613963
9
F
0.169704038
41
0.164599723
19.53125
vaginal
51
3570
864
650
non-smoker
cord blood non-exposed
GSM743286
non-smoker 228, cord blood
1
34.14921287
10
F
0.087563993
41
0.087563993
18.42403628
vaginal
45
2810
2100
600
non-smoker
cord blood non-exposed
GSM743287
non-smoker 231, cord blood
3
32.30937714
9
F
0.156431416
40
0.121901448
28.73174689
vaginal
50
3650
1500
750
non-smoker
cord blood non-exposed
GSM743288
passive smoker 232, cord blood
2
33.61806982
10
F
0.360452481
40
0.49
36.84424013
vaginal
49
3170
1452
650
passive smoker
cord blood passive smoker
GSM743289
passive smoker 234, cord blood
1
24.21355236
10
M
0.29
40
0.23
22.58324288
vaginal
48
3200
1440
600
passive smoker
cord blood passive smoker
GSM743290
non-smoker 236, cord blood
1
32.99657769
10
F
0.276288862
39
0.089358617
26.953125
vaginal
48
2980
1120
620
non-smoker
cord blood non-exposed
GSM743291
non-smoker 238, cord blood
2
32.08213552
10
M
0.133819485
41
0.110690542
20.64341393
vaginal
53
3950
2700
670
non-smoker
cord blood non-exposed
GSM743292
non-smoker 240, cord blood
3
30.69130732
10
F
0.23
38
0.334019804
17.44126398
vaginal
50
2880
576
620
non-smoker
cord blood non-exposed
GSM743293
non-smoker 241, cord blood
2
28.41615332
10
M
0.102899865
41
0.077977825
20.70081674
vaginal
46
2860
972
650
non-smoker
cord blood non-exposed
GSM743294
passive smoker 245, cord blood
6
30.92128679
10
M
1.18
39
1.45
22.09317005
vaginal
51
3600
1760
690
passive smoker
cord blood passive smoker
GSM743295
passive smoker 246, cord blood
1
27.40041068
9
M
0.16336322
41
0.133819485
19.72386588
caesarean
51
3370
1200
630
passive smoker
cord blood passive smoker
GSM743296
non-smoker 248, cord blood
1
34.52703628
10
F
0.150394684
40
0.123797136
25.60553633
vaginal
53
3690
1320
600
non-smoker
cord blood non-exposed
GSM743297
passive smoker 251, cord blood
2
34.00684463
8
M
0.337285997
36
0.139130944
19.00390835
caesarean
47
2840
1500
580
passive smoker
cord blood passive smoker
GSM743298
non-smoker 252, cord blood
4
33.87542779
10
F
0.294328957
39
0.225224379
26.98961938
caesarean
52
3770
2250
600
non-smoker
cord blood non-exposed
GSM743299
passive smoker 253, cord blood
unknown
48.95824778
10
M
0.115645287
39
0.154098255
22.48132907
caesarean
50
3600
1350
500
passive smoker
cord blood passive smoker
GSM743300
passive smoker 256, cord blood
1
25.93839836
10
M
1.570848085
41
1.088873153
21.23057202
vaginal
49
3500
1875
730
passive smoker
cord blood passive smoker
GSM743301
passive smoker 258, cord blood
3
32.48733744
10
F
1.758594711
35
1.405734212
25.51020408
vaginal
48
3490
1152
unknown
passive smoker
cord blood passive smoker
GSM743302
passive smoker 262, cord blood
2
25.5797399
9
M
0.25891947
39
0.379274063
21.51385851
caesarean
55
4550
1656
unknown
passive smoker
cord blood passive smoker
GSM743303
passive smoker 263, cord blood
2
30.55715264
8
F
1.355280925
37
1.411605772
24.05693475
vaginal
48
2650
720
unknown
passive smoker
cord blood passive smoker
GSM743304
non-smoker 265, cord blood
1
23.97809719
10
M
0.25891947
40
0.138443215
29.75778547
vaginal
51
3530
800
unknown
non-smoker
cord blood non-exposed
GSM743305
non-smoker 266, cord blood
2
32.15605749
10
F
0.232671906
38
0.295554811
18.37682515
vaginal
51
3250
1026
700
non-smoker
cord blood non-exposed
GSM743306
passive smoker 267, cord blood
6
35.08555784
10
F
0.266948684
42
0.16
22.03172568
caesarean
50
3080
1140
500
passive smoker
cord blood passive smoker
GSM743307
non-smoker 268, cord blood
2
32.11772758
10
F
0.251131757
40
0.199723227
19.10009183
vaginal
49
3040
1134
unknown
non-smoker
cord blood non-exposed
GSM743308
passive smoker 270, cord blood
1
34.33264887
9
M
1.462806942
39
0.953902455
34.14007572
caesarean
49
3370
1875
400
passive smoker
cord blood passive smoker
GSM743309
passive smoker 274, cord blood
1
24.28747433
9
M
0.248161846
39
0.204690319
19.70553242
vaginal
51
3400
880
unknown
passive smoker
cord blood passive smoker
GSM743310
non-smoker 284, cord blood
2
26.95687885
10
F
0.231164488
36
0.111989992
28.57796068
vaginal
48
2870
1000
unknown
non-smoker
cord blood non-exposed
GSM743311
non-smoker 287, cord blood
1
29.85626283
10
M
0.31015492
39
0.120224535
21.23057202
vaginal
47
2620
1071
unknown
non-smoker
cord blood non-exposed
GSM743312
passive smoker 289, cord blood
unknown
27.47980835
9
F
0.269123045
41
0.186844122
21.10726644
vaginal
53
4150
1188
unknown
passive smoker
cord blood passive smoker
GSM743313
non-smoker 291, cord blood
1
29.32511978
10
M
0.442229936
39
0.294828017
21.2585034
vaginal
49
3040
864
unknown
non-smoker
cord blood non-exposed
GSM743314
non-smoker 292, cord blood
2
35.59206023
10
M
0.650015266
38
0.796091983
21.50188649
vaginal
46
2890
800
unknown
non-smoker
cord blood non-exposed
GSM743315
passive smoker 293, cord blood
3
33.08966461
10
F
0.15
39
0.07
23.52941176
vaginal
50
3160
576
unknown
passive smoker
cord blood passive smoker
GSM743316
non-smoker 294, cord blood
3
35.00342231
10
M
0.124566153
40
0.123936452
21.7046498
vaginal
53
4720
1080
unknown
non-smoker
cord blood non-exposed
GSM743317
passive smoker 295, cord blood
3
30.29158111
10
F
0.24
37
0.308288342
25.86451247
vaginal
49
3720
640
700
passive smoker
cord blood passive smoker
GSM743318
non-smoker 299, cord blood
1
29.96303901
10
M
0.158418373
41
0.118628907
20.47826661
vaginal
50
3770
1794
unknown
non-smoker
cord blood non-exposed
GSM743319
non-smoker 300, cord blood
2
29.5523614
9
M
0.355287202
39
0.291914827
20.703125
vaginal
47
2570
810
unknown
non-smoker
cord blood non-exposed

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