Sample Set Annotation Tool: Comparison of gene expression profiles by CD3+CD4+ thymocytes derived from fetal and adult hematopoietic stem cells

Study Design
Assay
Sample Info
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Info
Samples
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Analysis

Purpose

Human fetal and adult hematopoietic stem cells (HSC) were obtained from fetal liver, fetal bone marrow (BM), and adult BM. These were injected into human fetal thymic implants in SCID-hu Thy/Liv mice (4-6 separate mice per HSC donor) and allowed to mature into single positive CD4+ (SP4) thymocytes over the course of 7-8 weeks. SP4 thymocytes from injected stem cells were subsequently sort-purified from thymic implants and gene expression was performed.

Hypothesis

Experimental Design

HSC from fetal (age 18-22 gestational weeks) and adult (age: 19-43 year old) HLA-A2+ donors were obtained from different tissues. After injection into human fetal thymic implants (SCID-hu Thy/Liv HLA-A2-) the cells were allowed to mature into thymocytes and sorted on the basis of HLA-A2+ expression and CD3+CD4+ (SP4) expression. 3 separate thymic implants were analyzed for each group.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

9 Samples Loaded: 9

Species

Human (Homo sapiens)

Sample Source

Fetal , CD4+ T cells , Adult

Disease

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	group	Cell type	Original cells	Group name
GSM616102	Fetal Liver SP4 thymic implant #1	fetal liver SP4	Sort Purifed Thymocytes	fetal liver HSC	fetal liver SP4
GSM616103	Fetal Liver SP4 thymic implant #2	fetal liver SP4	Sort Purifed Thymocytes	fetal liver HSC	fetal liver SP4
GSM616104	Fetal Liver SP4 thymic implant #3	fetal liver SP4	Sort Purifed Thymocytes	fetal liver HSC	fetal liver SP4
GSM616105	Fetal BM SP4 thymic implant #1	fetal bone marrow SP4	Sort Purifed Thymocytes	fetal bone marrow HSC	fetal bone marrow SP4
GSM616106	Fetal BM SP4 thymic implant #2	fetal bone marrow SP4	Sort Purifed Thymocytes	fetal bone marrow HSC	fetal bone marrow SP4

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Sample ID	!Sample Title	Group	Cell type	Original cells	Group name
GSM616102	Fetal Liver SP4 thymic implant #1	fetal liver SP4	Sort Purifed Thymocytes	fetal liver HSC	fetal liver SP4
GSM616103	Fetal Liver SP4 thymic implant #2	fetal liver SP4	Sort Purifed Thymocytes	fetal liver HSC	fetal liver SP4
GSM616104	Fetal Liver SP4 thymic implant #3	fetal liver SP4	Sort Purifed Thymocytes	fetal liver HSC	fetal liver SP4
GSM616105	Fetal BM SP4 thymic implant #1	fetal bone marrow SP4	Sort Purifed Thymocytes	fetal bone marrow HSC	fetal bone marrow SP4
GSM616106	Fetal BM SP4 thymic implant #2	fetal bone marrow SP4	Sort Purifed Thymocytes	fetal bone marrow HSC	fetal bone marrow SP4
GSM616107	Fetal BM SP4 thymic implant #3	fetal bone marrow SP4	Sort Purifed Thymocytes	fetal bone marrow HSC	fetal bone marrow SP4
GSM616108	Adult BM SP4 thymic implant #1	adult bone marrow SP4	Sort Purifed Thymocytes	adult bone marrow HSC	adult bone marrow SP4
GSM616109	Adult BM SP4 thymic implant #2	adult bone marrow SP4	Sort Purifed Thymocytes	adult bone marrow HSC	adult bone marrow SP4
GSM616110	Adult BM SP4 thymic implant #3	adult bone marrow SP4	Sort Purifed Thymocytes	adult bone marrow HSC	adult bone marrow SP4

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SIDRA