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Clinical

This SubSeries is part of SuperSeries GSE25119: Comparison of CD4+ T cells from human fetal and adult donors

Purpose

Human fetal and adult hematopoietic stem cells (HSC) were obtained from fetal liver, fetal bone marrow (BM), and adult BM. These were injected into human fetal thymic implants in SCID-hu Thy/Liv mice (4-6 separate mice per HSC donor) and allowed to mature into single positive CD4+ (SP4) thymocytes over the course of 7-8 weeks. SP4 thymocytes from injected stem cells were subsequently sort-purified from thymic implants and gene expression was performed.

Hypothesis

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Experimental Design

HSC from fetal (age 18-22 gestational weeks) and adult (age: 19-43 year old) HLA-A2+ donors were obtained from different tissues. After injection into human fetal thymic implants (SCID-hu Thy/Liv HLA-A2-) the cells were allowed to mature into thymocytes and sorted on the basis of HLA-A2+ expression and CD3+CD4+ (SP4) expression. 3 separate thymic implants were analyzed for each group.

Experimental Variables

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Controls

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Methods

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Additional Information

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Microarray
Affymetrix HG-U133_Plus_2
9 Samples Loaded: 9
Human (Homo sapiens)
Fetal , CD4+ T cells , Adult
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample Title group Cell type Original cells Group name
GSM616102 Fetal Liver SP4 thymic implant #1 fetal liver SP4 Sort Purifed Thymocytes fetal liver HSC fetal liver SP4
GSM616103 Fetal Liver SP4 thymic implant #2 fetal liver SP4 Sort Purifed Thymocytes fetal liver HSC fetal liver SP4
GSM616104 Fetal Liver SP4 thymic implant #3 fetal liver SP4 Sort Purifed Thymocytes fetal liver HSC fetal liver SP4
GSM616105 Fetal BM SP4 thymic implant #1 fetal bone marrow SP4 Sort Purifed Thymocytes fetal bone marrow HSC fetal bone marrow SP4
GSM616106 Fetal BM SP4 thymic implant #2 fetal bone marrow SP4 Sort Purifed Thymocytes fetal bone marrow HSC fetal bone marrow SP4
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Sample ID !Sample Title Group Cell type Original cells Group name
GSM616102
Fetal Liver SP4 thymic implant #1
fetal liver SP4
Sort Purifed Thymocytes
fetal liver HSC
fetal liver SP4
GSM616103
Fetal Liver SP4 thymic implant #2
fetal liver SP4
Sort Purifed Thymocytes
fetal liver HSC
fetal liver SP4
GSM616104
Fetal Liver SP4 thymic implant #3
fetal liver SP4
Sort Purifed Thymocytes
fetal liver HSC
fetal liver SP4
GSM616105
Fetal BM SP4 thymic implant #1
fetal bone marrow SP4
Sort Purifed Thymocytes
fetal bone marrow HSC
fetal bone marrow SP4
GSM616106
Fetal BM SP4 thymic implant #2
fetal bone marrow SP4
Sort Purifed Thymocytes
fetal bone marrow HSC
fetal bone marrow SP4
GSM616107
Fetal BM SP4 thymic implant #3
fetal bone marrow SP4
Sort Purifed Thymocytes
fetal bone marrow HSC
fetal bone marrow SP4
GSM616108
Adult BM SP4 thymic implant #1
adult bone marrow SP4
Sort Purifed Thymocytes
adult bone marrow HSC
adult bone marrow SP4
GSM616109
Adult BM SP4 thymic implant #2
adult bone marrow SP4
Sort Purifed Thymocytes
adult bone marrow HSC
adult bone marrow SP4
GSM616110
Adult BM SP4 thymic implant #3
adult bone marrow SP4
Sort Purifed Thymocytes
adult bone marrow HSC
adult bone marrow SP4

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