Sample Set Annotation Tool: Human MAIT and CD8++ cell development

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Microarray was used to compare gene expression between CD161++/CD161+/CD161-CD8+ T cells from human cord blood.

Hypothesis

Experimental Design

Lymphocytes from freshly obtained human cord blood samples were isolated by Ficoll density centrifugation. CD8+ T cells were purified by negative selection using magnetic beads and subsequently labelled with fluorescent antibodies prior to sorting using MoFlo MLS cell sorter (Dako).

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

17 Samples Loaded: 17

Species

Human (Homo sapiens)

Sample Source

CD8+ T cells , Adult , Neonatal

Disease

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	tissue	group	Cell type
GSM825517	CD161++CD8aa, biological rep 1	peripheral blood	CD161++CD8aa T cells	CD161++CD8aa T cells
GSM825518	CD161++CD8ab, biological rep 1	peripheral blood	CD161++CD8ab T cells	CD161++CD8ab T cells
GSM825519	CD161++CD8aa, biological rep 2	peripheral blood	CD161++CD8aa T cells	CD161++CD8aa T cells
GSM825520	CD161++CD8ab, biological rep 2	peripheral blood	CD161++CD8ab T cells	CD161++CD8ab T cells
GSM825521	CD161++CD8aa, biological rep 3	peripheral blood	CD161++CD8aa T cells	CD161++CD8aa T cells

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File

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Tissue	Group	Cell type	Cell subtype	Disease state
GSM825517	CD161++CD8aa, biological rep 1	peripheral blood	CD161++CD8aa T cells	CD161++CD8aa T cells		healthy
GSM825518	CD161++CD8ab, biological rep 1	peripheral blood	CD161++CD8ab T cells	CD161++CD8ab T cells		healthy
GSM825519	CD161++CD8aa, biological rep 2	peripheral blood	CD161++CD8aa T cells	CD161++CD8aa T cells		healthy
GSM825520	CD161++CD8ab, biological rep 2	peripheral blood	CD161++CD8ab T cells	CD161++CD8ab T cells		healthy
GSM825521	CD161++CD8aa, biological rep 3	peripheral blood	CD161++CD8aa T cells	CD161++CD8aa T cells		healthy
GSM825522	CD161++CD8ab, biological rep 3	peripheral blood	CD161++CD8ab T cells	CD161++CD8ab T cells		healthy
GSM825523	CD161++CD8aa, biological rep 4	peripheral blood	CD161++CD8aa T cells	CD161++CD8aa T cells		healthy
GSM825524	CD161++CD8ab, biological rep 4	peripheral blood	CD161++CD8ab T cells	CD161++CD8ab T cells		healthy
GSM826755	CD161++CD8+ T cells, biological repeat 1	CD8+ T cell	CD161++CD8+ T cells		CD161++CD8+ T cells
GSM826756	CD161+CD8+ T cells, biological repeat 1	CD8+ T cell	CD161+CD8+ T cells		CD161+CD8+ T cells
GSM826757	CD161-CD8+ T cells, biological repeat 1	CD8+ T cell	CD161-CD8+ T cells		CD161-CD8+ T cells
GSM826758	CD161++CD8+ T cells, biological repeat 2	CD8+ T cell	CD161++CD8+ T cells		CD161++CD8+ T cells
GSM826759	CD161+CD8+ T cells, biological repeat 2	CD8+ T cell	CD161+CD8+ T cells		CD161+CD8+ T cells
GSM826760	CD161-CD8+ T cells, biological repeat 2	CD8+ T cell	CD161-CD8+ T cells		CD161-CD8+ T cells
GSM826761	CD161++CD8+ T cells, biological repeat 3	CD8+ T cell	CD161++CD8+ T cells		CD161++CD8+ T cells
GSM826762	CD161+CD8+ T cells, biological repeat 3	CD8+ T cell	CD161+CD8+ T cells		CD16+-CD8+ T cells
GSM826763	CD161-CD8+ T cells, biological repeat 3	CD8+ T cell	CD161-CD8+ T cells		CD161-CD8+ T cells

Group Sets View in Gene Expression Browser

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Module Analysis

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