Sample Set Annotation Tool: Human Fetal and Adult Peripheral Naïve CD4+ T cells and CD4+CD25+ Treg cells

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

We compared differences in fetal and adult T cells by performing whole genome profiling on sort-purified T cells (naïve CD4+ and Treg cells) from human fetal specimens (18-22 gestational weeks) and adult specimens (age 25-40 years old). Fetal and Adult Naïve CD4+ T cells phenotype: CD3+CD4+CD45RA+CCR7+CD27+, Fetal and Adult CD4+CD25+ Treg phenotype: CD3+CD4+CD25bright

Hypothesis

Experimental Design

Four different groups were analyzed: Fetal Naïve CD4+ T cells, Adult Naïve CD4+ T cells, Fetal Treg cells, Adult Treg cells. For each group three independent donors were analyzed.

Experimental Variables

Controls

Methods

Fetal and Adult Naïve CD4+ T cells phenotype: CD3+CD4+CD45RA+CCR7+CD27+, Fetal and Adult CD4+CD25+ Treg phenotype: CD3+CD4+CD25bright

Additional Information

Expected Samples:

12 Samples Loaded: 12

Species

Human (Homo sapiens)

Sample Source

Adult , CD4+ T cells , Fetal

Disease

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	phenotype	Cell type	Group name
GSM616115	Fetal Naïve CD4+ T cells 1	CD3+CD4+CD45RA+CCR7+CD27+	Sort Purified T cells	fetal naive CD4+ T cells
GSM616116	Fetal Naïve CD4+ T cells 2	CD3+CD4+CD45RA+CCR7+CD27+	Sort Purified T cells	fetal naive CD4+ T cells
GSM616117	Fetal Naïve CD4+ T cells 3	CD3+CD4+CD45RA+CCR7+CD27+	Sort Purified T cells	fetal naive CD4+ T cells
GSM616118	Fetal CD4+CD25+ Treg 1	CD3+CD4+CD25bright	Sort Purified T cells	fetal Treg cells
GSM616119	Fetal CD4+CD25+ Treg 2	CD3+CD4+CD25bright	Sort Purified T cells	fetal Treg cells

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Sample ID	!Sample Title	Phenotype	Cell type	Group name
GSM616115	Fetal Naïve CD4+ T cells 1	CD3+CD4+CD45RA+CCR7+CD27+	Sort Purified T cells	fetal naive CD4+ T cells
GSM616116	Fetal Naïve CD4+ T cells 2	CD3+CD4+CD45RA+CCR7+CD27+	Sort Purified T cells	fetal naive CD4+ T cells
GSM616117	Fetal Naïve CD4+ T cells 3	CD3+CD4+CD45RA+CCR7+CD27+	Sort Purified T cells	fetal naive CD4+ T cells
GSM616118	Fetal CD4+CD25+ Treg 1	CD3+CD4+CD25bright	Sort Purified T cells	fetal Treg cells
GSM616119	Fetal CD4+CD25+ Treg 2	CD3+CD4+CD25bright	Sort Purified T cells	fetal Treg cells
GSM616120	Fetal CD4+CD25+ Treg 3	CD3+CD4+CD25bright	Sort Purified T cells	fetal Treg cells
GSM616121	Adult Naïve CD4+ T cells 1	CD3+CD4+CD45RA+CCR7+CD27+	Sort Purified T cells	adult naive CD4+ T cells
GSM616122	Adult Naïve CD4+ T cells 2	CD3+CD4+CD45RA+CCR7+CD27+	Sort Purified T cells	adult naive CD4+ T cells
GSM616123	Adult Naïve CD4+ T cells 3	CD3+CD4+CD45RA+CCR7+CD27+	Sort Purified T cells	adult naive CD4+ T cells
GSM616124	Adult CD4+CD25+ Treg 1	CD3+CD4+CD25bright	Sort Purified T cells	adult Treg cells
GSM616125	Adult CD4+CD25+ Treg 2	CD3+CD4+CD25bright	Sort Purified T cells	adult Treg cells
GSM616126	Adult CD4+CD25+ Treg 3	CD3+CD4+CD25bright	Sort Purified T cells	adult Treg cells

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