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Clinical

This SubSeries is part of SuperSeries GSE25119: Comparison of CD4+ T cells from human fetal and adult donors

Purpose

We compared differences in fetal and adult T cells by performing whole genome profiling on sort-purified T cells (naïve CD4+ and Treg cells) from human fetal specimens (18-22 gestational weeks) and adult specimens (age 25-40 years old). Fetal and Adult Naïve CD4+ T cells phenotype: CD3+CD4+CD45RA+CCR7+CD27+, Fetal and Adult CD4+CD25+ Treg phenotype: CD3+CD4+CD25bright

Hypothesis

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Experimental Design

Four different groups were analyzed: Fetal Naïve CD4+ T cells, Adult Naïve CD4+ T cells, Fetal Treg cells, Adult Treg cells. For each group three independent donors were analyzed.

Experimental Variables

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Controls

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Methods

Fetal and Adult Naïve CD4+ T cells phenotype: CD3+CD4+CD45RA+CCR7+CD27+, Fetal and Adult CD4+CD25+ Treg phenotype: CD3+CD4+CD25bright

Additional Information

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Microarray
Affymetrix HG-U133_Plus_2
12 Samples Loaded: 12
Human (Homo sapiens)
Fetal , Adult , CD4+ T cells
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample Title phenotype Cell type Group name
GSM616115 Fetal Naïve CD4+ T cells 1 CD3+CD4+CD45RA+CCR7+CD27+ Sort Purified T cells fetal naive CD4+ T cells
GSM616116 Fetal Naïve CD4+ T cells 2 CD3+CD4+CD45RA+CCR7+CD27+ Sort Purified T cells fetal naive CD4+ T cells
GSM616117 Fetal Naïve CD4+ T cells 3 CD3+CD4+CD45RA+CCR7+CD27+ Sort Purified T cells fetal naive CD4+ T cells
GSM616118 Fetal CD4+CD25+ Treg 1 CD3+CD4+CD25bright Sort Purified T cells fetal Treg cells
GSM616119 Fetal CD4+CD25+ Treg 2 CD3+CD4+CD25bright Sort Purified T cells fetal Treg cells
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Sample ID !Sample Title Phenotype Cell type Group name
GSM616115
Fetal Naïve CD4+ T cells 1
CD3+CD4+CD45RA+CCR7+CD27+
Sort Purified T cells
fetal naive CD4+ T cells
GSM616116
Fetal Naïve CD4+ T cells 2
CD3+CD4+CD45RA+CCR7+CD27+
Sort Purified T cells
fetal naive CD4+ T cells
GSM616117
Fetal Naïve CD4+ T cells 3
CD3+CD4+CD45RA+CCR7+CD27+
Sort Purified T cells
fetal naive CD4+ T cells
GSM616118
Fetal CD4+CD25+ Treg 1
CD3+CD4+CD25bright
Sort Purified T cells
fetal Treg cells
GSM616119
Fetal CD4+CD25+ Treg 2
CD3+CD4+CD25bright
Sort Purified T cells
fetal Treg cells
GSM616120
Fetal CD4+CD25+ Treg 3
CD3+CD4+CD25bright
Sort Purified T cells
fetal Treg cells
GSM616121
Adult Naïve CD4+ T cells 1
CD3+CD4+CD45RA+CCR7+CD27+
Sort Purified T cells
adult naive CD4+ T cells
GSM616122
Adult Naïve CD4+ T cells 2
CD3+CD4+CD45RA+CCR7+CD27+
Sort Purified T cells
adult naive CD4+ T cells
GSM616123
Adult Naïve CD4+ T cells 3
CD3+CD4+CD45RA+CCR7+CD27+
Sort Purified T cells
adult naive CD4+ T cells
GSM616124
Adult CD4+CD25+ Treg 1
CD3+CD4+CD25bright
Sort Purified T cells
adult Treg cells
GSM616125
Adult CD4+CD25+ Treg 2
CD3+CD4+CD25bright
Sort Purified T cells
adult Treg cells
GSM616126
Adult CD4+CD25+ Treg 3
CD3+CD4+CD25bright
Sort Purified T cells
adult Treg cells

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