Sample Set Annotation Tool: Gene Expression Profile during human CD4+ T cell differentiation

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Analysis of CD4+ T cell differentiation by expression profiling of subpopulations of CD4+ cells representing 5 successive stages of differentiation: intrathymic T progenitors, double positive thymocytes, single positive thymocytes, naïve T cells from cord blood, and naïve T cells from adult blood.

Hypothesis

Experimental Design

Subpopulations of human fetal thymocyte and circulating naïve T cells were obtained through FACS sorting, including CD3-CD4+CD8- intrathymic T progenitor cells (ITTP), CD3intCD4+CD8+ "double positive" thymocytes (DP), CD3highCD4+CD8- "single positive" thymocytes (SP4), CD3+CD4+CD8-CD45RA+CD62L+ naïve T cells from cord blood (CB4+), and CD3+CD4+CD8-CD45RA+CD62L+ naïve T cells from adult blood (AB4+).

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

15 Samples Loaded: 15

Species

Human (Homo sapiens)

Sample Source

Adult , Fetal , CD4+ T cells , Neonatal

Disease

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	Group name
GSM24511	ITTP-1A	ITTP
GSM24609	ITTP-2A	ITTP
GSM24610	ITTP-3A	ITTP
GSM24611	DP-1A	DP
GSM24612	DP-2A	DP

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File
GSE1460 GPL96 modified.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Group name
GSM24511	ITTP-1A	ITTP
GSM24609	ITTP-2A	ITTP
GSM24610	ITTP-3A	ITTP
GSM24611	DP-1A	DP
GSM24612	DP-2A	DP
GSM24613	SP4-1A	SP4
GSM24614	SP4-2A	SP4
GSM24615	SP4-3A	SP4
GSM24616	CB4-1A	CB4+
GSM24617	CB4-2A	CB4+
GSM24618	AB4-1A	AB4+
GSM24619	AB4-2A	AB4+
GSM24620	AB4-3A	AB4+
GSM24621	TSC-1A	TSC
GSM24622	TSC-2A	TSC

Group Sets View in Gene Expression Browser

Name
All Samples	Default	View
Test		View
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Module Analysis

Group Set
All Samples
Test

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