Gene Expression Profile during human CD4+ T cell differentiation - GSE1460 (GPL97)
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| Description | ITTP, fetal CD3-CD4+CD8- intrathymic T progenitor cells DP, fetal CD3intCD4+CD8+ "double positive" thymocytes SP4, fetal CD3highCD4+CD8- “single positive” thymocytes TSC, fetal thymic stromal cells |
| Purpose | Analysis of CD4+ T cell differentiation by expression profiling of subpopulations of CD4+ cells representing 5 successive stages of differentiation: intrathymic T progenitors, double positive thymocytes, single positive thymocytes, naïve T cells from cord blood, and naïve T cells from adult blood. |
| Experimental Design | Subpopulations of human fetal thymocyte and circulating naïve T cells were obtained through FACS sorting, including CD3-CD4+CD8- intrathymic T progenitor cells (ITTP), CD3intCD4+CD8+ "double positive" thymocytes (DP), CD3highCD4+CD8- "single positive" thymocytes (SP4), CD3+CD4+CD8-CD45RA+CD62L+ naïve T cells from cord blood (CB4+), and CD3+CD4+CD8-CD45RA+CD62L+ naïve T cells from adult blood (AB4+). |
| Platform | Affymetrix HG-U133B |
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