This SuperSeries is composed of the following SubSeries:

GSE25085 Comparison of gene expression profiles by CD3+CD4+ thymocytes derived from fetal and adult hematopoietic stem cells

GSE25087 Human Fetal and Adult Peripheral Naïve CD4+ T cells and CD4+CD25+ Treg cells

Human fetal and adult hematopoietic stem cells (HSC) were obtained from fetal liver, fetal bone marrow (BM), and adult BM. These were injected into human fetal thymic implants in SCID-hu Thy/Liv mice (4-6 separate mice per HSC donor) and allowed to mature into single positive CD4+ (SP4) thymocytes over the course of 7-8 weeks. SP4 thymocytes from injected stem cells were subsequently sort-purified from thymic implants and gene expression was performed. in additon, We compared differences in fetal and adult T cells by performing whole genome profiling on sort-purified T cells (naïve CD4+ and Treg cells) from human fetal specimens (18-22 gestational weeks) and adult specimens (age 25-40 years old). Fetal and Adult Naïve CD4+ T cells phenotype: CD3+CD4+CD45RA+CCR7+CD27+, Fetal and Adult CD4+CD25+ Treg phenotype: CD3+CD4+CD25bright

HSC from fetal (age 18-22 gestational weeks) and adult (age: 19-43 year old) HLA-A2+ donors were obtained from different tissues. After injection into human fetal thymic implants (SCID-hu Thy/Liv HLA-A2-) the cells were allowed to mature into thymocytes and sorted on the basis of HLA-A2+ expression and CD3+CD4+ (SP4) expression. 3 separate thymic implants were analyzed for each group.

Four different groups were analyzed: Fetal Naïve CD4+ T cells, Adult Naïve CD4+ T cells, Fetal Treg cells, Adult Treg cells. For each group three independent donors were analyzed.

Group Set:

FL SP4, CD3+CD4+ thymocytes derived from fetal liver HSC

FBM SP4, CD3+CD4+ thymocytes derived from fetal bone marrow HSC

ABM SP4, CD3+CD4+ thymocytes derived from adult bone marrow HSC

Fetal CD4+, sort-purified CD3+CD4+CD45RA+CCR7+CD27+ naïve T cells of fetal origin

Adult CD4+, sort purified CD3+CD4+CD45RA+CCR7+CD27+ naïve T cells from adults

Fetal Treg, sort-purified CD3+CD4+CD25bright Treg cells of fetal origin

Adult Treg, sort-purified CD3+CD4+CD25bright Treg cells from adults