HSC1, Hematopoietic stem cell CD133+ CD34dim

HSC2, Hematopoietic stem cell CD38- CD34+

CMP, Common myeloid progenitor

MEP, Megakaryocyte/erythroid progenitor

ERY1, Erythroid CD34+ CD71+ GlyA-

ERY2, Erythroid CD34- CD71+ GlyA-

ERY3, Erythroid CD34- CD71+ GlyA+

ERY4, Erythroid CD34- CD71lo GlyA+

ERY5, Erythroid CD34- CD71- GlyA+

MEGA1, Colony Forming Unit-Megakaryocytic

MEGA2, Megacaryocyte

DENDa1, Plasmacytoid dendritic cell

DENDa2, Myeloid dendritic cell

GMP, Granulocyte/monocyte progenitor

GRAN1, Colony Forming Unit-Granulocyte

GRAN2, Granulocyte (Neutrophilic Metamyelocyte)

GRAN3, Granulocyte (Neutrophil)

MONO1, Colony Forming Unit-Monocyte

MONO2, Monocyte

BASO1, Basophil

EOS2, Eosinophil

Pre-BCELL2, Early B cell

Pre-BCELL3, Pro-B cell

BCELLa1, Naive B cell

BCELLa2, Mature B cell, able to class switch

BCELLa3, Mature B cell

BCELLa4, Mature B cell, class switched

NKa1, Mature NK cell_CD56- CD16+ CD3-

NKa2, Mature NK cell_CD56+ CD16+ CD3-

NKa3, Mature NK cell CD56- CD16- CD3-

NKa4, NKT cell

TCELL1, CD8+ effector memory RA

TCELL2, Naive CD8+ T cell

TCELL3, CD8+ effector memory cell

TCELL4, CD8+ central memory

TCELL6, Naive CD4+ T cell

TCELL7, CD4+ effector memory cell

TCELL8, CD4+ central memory

NKa1-4 as well as DENDa1 and DENDa2 cells were isolated from adult peripheral blood, other cell populations were isolated from cord blood.

Tremendous progress has been made in identifying individual transcription factors that regulate hematopoietic differentiation, but many aspects of the global architecture of hematopoiesis remain unknown.

Here, we profiled gene expression in 38 distinct purified populations of human hematopoietic cells and used probabilistic models of gene expression patterns and sequence-based analysis of motifs in gene promoters to decipher ...

We defined 38 distinct hematopoietic cell states based on cell surface marker expression, representing hematopoietic stem and progenitor cells, terminally differentiated cells, and intermediate states. For each state, we ...

38 Hematopoietic cell populations were purified by flow cytometry and analyzed by gene expression profiling. Adult peripheral blood was isolated, terminally differentiated and antigen activated. Other cell populations were isolated from cord blood. Cells that were studied include hematopoietic stem cell (HSC1,2), common myeloid progenitor (CMP), megakaryocyte/erythroid progenitor (MEP), erythroid cells (ERY1-5), CFU-MK (MEGA1),